Judging Category
Basic or Experimental Research
Student Rank
Sophomore
College
Sciences and Mathematics
Faculty Sponsor
Jianfeng Xu - jxu@astate.edu
Description
Plant-based systems are becoming an important method for producing pharmaceutical proteins because they can be more cost-effective, and safer compared to traditional production methods which commonly use mammalian cells. This project aims to develop a transgenic plant cell system that can produce a pharmaceutical protein using molecular cloning techniques. A transgenic plant cell has the DNA of another plant integrated into its genome, altering its gene expression to produce different proteins. To begin, a plasmid carrying the gene of interest and the DNA components needed for expression will be designed. The plasmid will then be used to introduce the gene into plant cells. Following transformation, the cells will be screened to confirm gene integration. Cells confirmed to carry the gene will then be analyzed to determine whether they are expressing the proper genes and producing the target protein. Successful expression in these cells will contribute to the development of more efficient and cost-effective methods for generating pharmaceutical proteins.
Disciplines
Biotechnology | Cell Biology | Molecular Genetics | Pharmaceutical Preparations | Plant Biology
License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Maria Gambaro. (2026). PER8–EGFP Expression in BY2 Plant Cell System. Create@State Research Poster, Arkansas State University
Included in
Biotechnology Commons, Cell Biology Commons, Molecular Genetics Commons, Pharmaceutical Preparations Commons, Plant Biology Commons
Transgenic Plant Cells for the Cost-Effective Production of Pharmaceutical Proteins
Plant-based systems are becoming an important method for producing pharmaceutical proteins because they can be more cost-effective, and safer compared to traditional production methods which commonly use mammalian cells. This project aims to develop a transgenic plant cell system that can produce a pharmaceutical protein using molecular cloning techniques. A transgenic plant cell has the DNA of another plant integrated into its genome, altering its gene expression to produce different proteins. To begin, a plasmid carrying the gene of interest and the DNA components needed for expression will be designed. The plasmid will then be used to introduce the gene into plant cells. Following transformation, the cells will be screened to confirm gene integration. Cells confirmed to carry the gene will then be analyzed to determine whether they are expressing the proper genes and producing the target protein. Successful expression in these cells will contribute to the development of more efficient and cost-effective methods for generating pharmaceutical proteins.
