Date of Award
1-10-2011
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Greg Phillips
Second Advisor
Kim Pittcock
Committee Members
Steven Green
Call Number
ISBN 9781124350813
Abstract
The objective of this study was to test the BDS (Dunstan and Short, 1977) basal salt formulation as an exemplary medium choice for in vitro applications utilizing rice, maize, soybean, cotton, onion, tobacco, muscadine, raspberry, and gerbera. The applications included: callus growth, plant regeneration, hairy root growth, production of secondary metabolites, and micropropagation. Two commonly used basal salt formulations MS (Murashige and Skoog, 1962) and B5 (Gamborg et al., 1968) were included as treatments in the experiments along with BCA, a version of BDS with MS level of calcium. BDS or BCA were comparable or superior to MS and B5 media in the majority of the test systems for callus growth, plant regeneration, micropropagation, and hairy root biomass. Metabolite productivity was superior on BCA in one of two test systems. BDS or BCA are recommended as reliable alternative basal media for a range of plant species and in vitro applications.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Greenway, Michael Brian, "BDS: An Alternative Medium for Regeneration and Growth of Multiple Plant Species In Vitro" (2011). Student Theses and Dissertations. 972.
https://arch.astate.edu/all-etd/972
