Date of Award
1-5-2012
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Malathi Srivatsan
Committee Members
Anne Grippo; Syed Ali
Call Number
LD251 .A566t 2011 P52
Abstract
Nanoparticles (NPs), materials fabricated in nanosize, are being increasingly used due to their unique applications. The potential risks from inhaling NPs and the interaction of inhaled NPs with the peripheral nervous system in rat were determined in this study by investigating 1) retrograde transport of inhaled NP's to the Nodose Ganglion (NDG) neurons, 2) NDG neuronal interaction with NPs in vitro, and 3) the fluid kinetics of NPs in cell sustaining media. Intratracheal intubation and instilling fluorescent NPs with and without a tracer dye (SP-DiOC) showed that NPS were transported from nerve endings in airway to neurons. Exposing NDG neurons to quantum dots (highly fluorescent NPs) in cell culture followed by pulse-chase, time-lapse microscopy, TEM and SEM revealed intake of NPs by neurons. Fluorescent correlation spectroscopic analysis of fluid kinetics of QDs in cell-sustaining medium showed aggregation of NPs in medium and related reduction in diffusion.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Pingel, Caleb Joseph, "Nanomaterials And Cellular Interaction in the Nervous System" (2012). Student Theses and Dissertations. 907.
https://arch.astate.edu/all-etd/907
