Date of Award
3-21-2019
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Elizabeth Hood
Second Advisor
Jiangfeng Xu
Committee Members
Gregory Phillips; Steven Green
Call Number
LD 251 .A566t 2019 V34
Abstract
Switchgrass (Panicum virgatum) is a perennial C4 grass that has been most extensively studied as a dedicated bioenergy crop. However, efficient conversion of switchgrass biomass to biofuels has been hampered by biomass recalcitrance. Genetic modification of the plant cell wall represents a promising solution to overcoming this problem. The goal of this project is to leverage an innovative strategy, the hydroxyproline (Hyp)-O-glycosylation "code", for de novo design and engineering in switchgrass of novel designer biopolymers (DBPs) to facilitate cell wall reconstruction. The Hyp-O-glycosylation of the engineered DBPs was characterized. The results from this study show; embryogenic switchgrass calli were successfully generated, the engineered recombinant HypGP tagged EGFPs {(SP)32-EGFP and (SP4)18-EGFP} were Hyp-O-glycosylated in switchgrass and rice, in whole plants or cultured cells, the Hyp-O-glycosylation of the HypGP tag can be performed by monocot plants the same as in dicot plants and no phenotypic significant difference of transgenic plants was observed.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Verma, Neha, "Hydroxyproline-O-Glycosylation in Monocot Plants and Its Application in Cell Wall Engineering" (2019). Student Theses and Dissertations. 487.
https://arch.astate.edu/all-etd/487
