Date of Award
11-30-2018
Document Type
Thesis
Degree Name
Molecular Bioscience, MS
First Advisor
Anne Grippo
Committee Members
Kris Biondolillo; Susan Motts
Call Number
LD 251 .A566t 2018 S53
Abstract
Differentiation of neural stem cells (NSCs) into large a number of oligodendrocytes (ODCs) for transplantation therapy has been a challenge. Evidence indicates that NSC differentiation is influenced by the interaction of the stem cells and their extracellular environment such as extracellular matrix (ECM), and soluble factors, such as growth factors and hormones. We tested if (1) well planned sequential addition of a combination of soluble factors increase the population of NSCs differentiating into ODCs and (2) a natural ECM (e.g, matrigel) promotes ODC differentiation better than poly-D-lysine (PDL). Embryonic rat NSCs were propagated in the presence of basic fibroblast growth factor, platelet derived growth factor alpha and differentiated on matrigel and PDL in the presence of triiodothyronine for ten days. Immunocytochemistry and fluorescence microscopy showed that 91.8%±4.2% of NSCs were differentiated into ODCs on matrigel compared to those on PDL (19.3%±5.8%). Further, we co-cultured hippocampal neurons and newly differentiated ODCs for three weeks to see if newly differentiated ODCs form myelin sheath. Our results showed that ODCs expressed myelin basic protein and formed myelin segments in week (WK) one. The myelin segments significantly increased as the culture progressed through WK2 and WK3.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Sharma, Krishna Deo, "Differentiation Of Oligodendrocytes for Myelination in the Nervous System" (2018). Student Theses and Dissertations. 461.
https://arch.astate.edu/all-etd/461
