Date of Award
8-14-2020
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Fabricio Medina-Bolivar
Committee Members
Argelia Lorence; Asela Wijeratne
Call Number
LD 251 .A566t 2020 R53
Abstract
Peanut (Arachis hypogaea) is a prolific producer of prenylated stilbenoids that may confer health benefits in humans by their antioxidant activity. Therefore, the objective of this study was to use peanut hairy roots as a bioproduction system of extracts enriched with prenylated stilbenoids and evaluate their antioxidant activity in vitro. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay suggested that the whole extract and pure prenylated stilbenoids exhibit antioxidant activity. High-performance liquid chromatography combined with DPPH identified arachidin-1, arachidin-3, and arachidin-5 as the principle antioxidants in the extract. The DPPH assay revealed that the arachidin portion of the extract contains greater antioxidant capacity than the resveratrol portion at lower doses. A cellular antioxidant assay based on 2′,7′- dichlorodihydrofluorescein diacetate staining revealed that the whole extract potentially attenuated hydrogen peroxide-induced free radical production. The robust antioxidant activity of the extract and pure stilbenoids may have profound implications in human health and research.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Roberto, Patrick, "Antioxidant Characterization of Peanut Hairy Root Extracts Enriched with Prenylated Stilbenoids" (2020). Student Theses and Dissertations. 382.
https://arch.astate.edu/all-etd/382
