Date of Award
9-1-2011
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Maureen Dolan
Committee Members
Carole Cramer; Jiangfeng Xu
Call Number
LD251 .A566t 2011 W95
Abstract
Plants have emerged as cost-effective, efficient factories to produce foreign proteins of pharmaceutical importance. Agrobacterium-mediated transient expression provides a rapid and simple plant system for production but is often linked with low protein recovery. Plant-specific hydroxyproline (Hyp)-O-glycosylation has been demonstrated to increase protein stability and solubility (termed Hyp-Glyco technology). To test if Hyp-glycans attached to a target protein can improve overall transiently expressed protein yields, EGFP reporter protein was expressed as fusion with a 5 or 32 repeats of Ser-Pro motif ((SP)5 or (SP)32 ) that directs extensive Hyp-O-glycosylation. My thesis project has provided the first demonstration that the Hyp-O-glcosylation occurs on recombinant proteins in a transient plant expression system. We confirmed that Hyp-Glyco technology is able to offer an opportunity for improved accumulation, recovery and stability of transient plant-produced recombinant proteins. Furthermore, the plant cell culture was used as an appropriate system to study the Hyp-O-glycosylation process in plants.
Rights Management
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Wu, Di, "Expression Of Hyp-O-Glycan Modified Protein in Plant Using Transient and Cell Culture Platforms" (2011). Student Theses and Dissertations. 946.
https://arch.astate.edu/all-etd/946
