Date of Award
1-3-2012
Document Type
Thesis
Degree Name
Chemistry, MS
First Advisor
Argelia Lorence
Committee Members
Maureen Dolan; Richard Warby
Call Number
LD251 .A566t 2011 Y37
Abstract
Human interleukin-12 (hIL-12) has been studied as a potential therapeutic factor in the immune response against viral disease and cancer. Plants are a promising platform for the production of complex mammalian proteins, as they can produce large amounts of functional polypeptides free of animal pathogens, and protein production can be increased to agricultural scale in a relatively short period of time. The presence of vitamin C (ascorbic acid, AsA) has been reported to have a positive effect on recombinant protein recovery. In a previous study we have demonstrated the favorable impact of the exogenous addition of AsA on the accumulation and recovery of hIL-12 synthesized in a Nicotiana benthamiana-based transient expression system. Based on these results, we hypothesized that the stable transformation of two key genes encoding proteins that will lead to enhanced levels of AsA and hIL-12 production will lead to plants that will produce and accumulate higher levels of hIL-12. To prove our hypothesis, stable Arabidopsis thaliana plants derived from crosses of plants over-expressing hIL 12 and AsA enhancing genes (myo-inositol oxygenase or AtMIOX4, and glucuronate reductase or AtGlcUR) were generated and characterized. Our results demonstrate a positive impact of elevated AsA content on hIL-12 production and recovery as the crosses (AtMIOX4 x hIL-12) accumulated 70% more hIL-12 compared to the parent line solely expressing this cytokine.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Yactayo-Chang, Jessica Patricia, "Stable Coexpression of Vitamin C Enhancing Genes for Improved Production of a Recombinant Therapeutic Protein, Hil-12, In Arabidopsis Thaliana" (2012). Student Theses and Dissertations. 905.
https://arch.astate.edu/all-etd/905
