Date of Award
8-11-2018
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Fabricio Medina-Bolivar
Committee Members
Elizabeth Hood; Maureen Dolan
Call Number
LD 251 .A566t 2018 A95
Abstract
Stilbenoids are phenolic compounds which not only serve as phytoalexins but also exhibit biological activities important to human health, including anticancer, anti-inflammatory and neuroprotective properties in vitro. However, some stilbenoids, such as the non prenylated stilbenoid resveratrol, have poor bioavailability limiting their application in vivo. Therefore, the goal of this project was to express a peanut stilbenoid prenyltransferase (i.e. AhR4DT-1) in muscadine grape to convert resveratrol to the prenylated stilbenoid arachidin-2, a potentially more bioavailable stilbenoid than resveratrol. Four hairy root lines of muscadine grape were developed via transformation with an engineered Agrobacterium rhizogenes harboring a peanut stilbenoid prenyltransferase. The prenyltransferase enzyme activity was detected in the crude extract of transgenic hairy root lines. Following 96 hours elicitation, stilbenoids were analyzed by high performance liquid chromatography (HPLC) and arachidin-2 was detected only in the medium of transgenic hairy roots and its identity was confirmed by liquid chromatography mass spectrometry (LC-MS).
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Azim, Mohammad Fazle, "Ectopic Expression of a Stilbenoid Prenyltransferase from Peanut in Hairy Roots of Muscadine Grape" (2018). Student Theses and Dissertations. 503.
https://arch.astate.edu/all-etd/503
