Date of Award

8-11-2018

Document Type

Thesis

Degree Name

Biology, MS

First Advisor

Fabricio Medina-Bolivar

Committee Members

Elizabeth Hood; Maureen Dolan

Call Number

LD 251 .A566t 2018 A95

Abstract

Stilbenoids are phenolic compounds which not only serve as phytoalexins but also exhibit biological activities important to human health, including anticancer, anti-inflammatory and neuroprotective properties in vitro. However, some stilbenoids, such as the non prenylated stilbenoid resveratrol, have poor bioavailability limiting their application in vivo. Therefore, the goal of this project was to express a peanut stilbenoid prenyltransferase (i.e. AhR4DT-1) in muscadine grape to convert resveratrol to the prenylated stilbenoid arachidin-2, a potentially more bioavailable stilbenoid than resveratrol. Four hairy root lines of muscadine grape were developed via transformation with an engineered Agrobacterium rhizogenes harboring a peanut stilbenoid prenyltransferase. The prenyltransferase enzyme activity was detected in the crude extract of transgenic hairy root lines. Following 96 hours elicitation, stilbenoids were analyzed by high performance liquid chromatography (HPLC) and arachidin-2 was detected only in the medium of transgenic hairy roots and its identity was confirmed by liquid chromatography mass spectrometry (LC-MS).

Rights Management

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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