Date of Award
8-11-2018
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Brett Savary
Committee Members
Steven Green; Tina Gray Teague; Xu Jianfeng
Call Number
LD 251 .A566t 2018 K36
Abstract
The purpose of this investigation was to isolate arabinoxylan (AX) from rice bran fiber and characterize its structural properties with enzyme and chromatographic techniques. The hypothesis was that glycoside hydrolase family 11 endo-xylanase (GH11-XYN) action on rice bran AX would generate oligosaccharide profiles that are distinguishable from other cereal species. The Specific Objectives addressed were to: 1) Extract AX fractions from rice bran fiber and determine yield and compositions. 2) Treat rice bran AX fractions with defined XYNs, and then characterize the enzyme-product profiles. Results showed that GH11-XYN generated large fragments from rice AX (average degree of polymerization 25 to 36) compared to the abundant short AXOs observed for rye and wheat AX (degree of polymerization 4 to 6). The rice AX fragments showed more than a double degree of substitution. This study establishes the means for preparing AX and enzymatically-generated products from rice bran fiber.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Kandanool, Divya, "Preparation and Characterization of Arabinoxylan from Rice Bran Fiber" (2018). Student Theses and Dissertations. 497.
https://arch.astate.edu/all-etd/497
