Date of Award
11-28-2018
Document Type
Dissertation
Degree Name
Molecular Biosciences, Ph.D.
First Advisor
Gregory Phillips
Committee Members
Carole Cramer; Giuliana Medrano; Glenn Bowers; Jianfeng Xu
Call Number
LD 251 .A566d 2018 G36
Abstract
Food is a constant world demand, and soybeans are among the most complete food crops which have fed people since its domestication. Unfortunately, soybean is a difficult crop to work within the biotechnology laboratory. Soybean androgenesis has been a challenging process yet to be advanced significantly after years of attempts. There have been some minor advances, for instance, reports of a 2% induction rate in anther culture, formation of roots, rare shoots, but only a handful of plantlets that did not survive to maturity. This dissertation compiles these efforts in the context of developing a pyramidal stressors model for androgenesis from soybeans and perhaps other legumes. Here we report the use of 10°C day /8°C night for 3 days plus 4°C overnight pretreatment of donor plants, a series of incubation temperatures while identifying 11°C that works best, nitrogen starvation medium (with up to 25% induction frequencies), a pH and osmoticum screening that showed to be permissive across tested parameters. Finally, the application of the anther culture protocol adapted to microspore cultures resulted in more than 90% response for sustained cell divisions with occasional formation of putative embryos. This last set of results has supported the use of microspore cultures as a new platform for soybean androgenesis.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Garda, Martina, "Soybean Androgenesis" (2018). Student Theses and Dissertations. 481.
https://arch.astate.edu/all-etd/481
