Date of Award
5-25-2019
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Gregory Phillips
Committee Members
Jianfeng Xu; Steven Green
Call Number
LD 251 .A566t 2019 R37
Abstract
Camelina sativa L. has potential as a winter oilseed crop to produce edible oils, livestock feed, biodiesel and jet fuel. Agrobacterium-mediated floral dip transformation of Camelina was performed to transfer the AtMIOX4 gene (Myo-inositol oxygenase from Arabidopsis thaliana) into Camelina as a means to improve agronomic characters. Three Agrobacterium strains LBA4404, GV3101 and EHA105 were used to transform Camelina sativa cultivar ‘Celine’. Recovered seeds were screened for expression of antibiotic resistance marker genes with only 2% survival of putative transgenic plants. Direct PCR analysis for the AtMIOX4 insertion was used to screen putative transgenic plants, giving an average of 10% positive transformants from antibiotic-screened plants, for an overall frequency of 0.2% confirmed transgenic lines. Some transgenic lines had up to three times as much foliar ascorbate content compared to the wild type, indicating a phenotypic alteration. These high ascorbate lines may provide a basis for improving agronomic characters in Camelina.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Rao, Naina Narayan, "Floral Dip Transformation of Camelina Sativa L. With A Myo-Inositol Oxygenase Gene to Potentially Improve Oil Yield" (2019). Student Theses and Dissertations. 455.
https://arch.astate.edu/all-etd/455
