Date of Award
10-2-2019
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Elizbeth Hood
Committee Members
Jennifer Bouldin; Stephen Grace
Call Number
LD 251 .A566t 2019 B97
Abstract
Contamination of the environment directly impacts animal, plant, and human health and agricultural production. A potential solution this project is offering incorporates the use of enzymes from fungi; manganese peroxidase and laccase. Two formulations of recombinant manganese peroxidase (MnP) from corn and commercial laccase have been tested on synthetic dyes and atrazine in an aqueous environment. Laccase utilizes oxygen when degrading these pollutants. All dyes were degraded to some extent by both enzymes. The products from the enzymatic reactions of one dye, Methyl Orange were analyzed with High Performance Liquid Chromatography (HPLC). The HPLC showed that there were products of degradation. Methyl Orange products underwent toxicity testing with fat head minnows to determine the change in toxicity of the Methyl Orange dye after treatment. The testing showed that the Methyl Orange dye’s toxicity was nontoxic before treatment and the treatment did not improve its toxicity.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Byrd, Joshua, "Plant-Produced Oxidation/Reduction Enzymes as Bioremediation Agents" (2019). Student Theses and Dissertations. 405.
https://arch.astate.edu/all-etd/405
