Date of Award
8-16-2022
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Guolei Zhou
Committee Members
Jennifer Xie; Jianfeng Xu
Call Number
LD 251 .A566t 2022 A33
Abstract
Cyclase-Associated Protein 1 (CAP1) is an actin-regulating protein that promotes actin dynamics and is also involved in cell adhesion and proliferation as well as implicated in human cancers. We previously found that phosphorylation at the Ser307/Ser309 tandem regulatory site on CAP1 is critical for CAP1 functions in breast and pancreatic cancer cells. We silenced CAP1 through RNAi in HCT116 and SW480 colon cancer cells as confirmed in Western blotting. We have found that depletion of CAP1 led to reduced FAK (Focal Adhesion Kinase) activity and matrix adhesion in SW480 cells, while it stimulated FAK activity and cell adhesion in HCT116 cells. Our findings support that both Rap1 and FAK play a role in CAP1 function in cell adhesion. In summary, we have discovered a novel pathway where CAP1 mediates signals from cAMP to regulate RAP1 activity and cell adhesion through PKA and Epac in HCT116 and SW480 cell lines.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Akana, Lokesh Kalki Mani Sai, "Cyclase-Associated Protein 1 (CAP1) In Cyclic Adenosine Monophosphate (CAMP) and Protein Kinase C (PKC) Signals to Regulate Cancer Cell Functions" (2022). Student Theses and Dissertations. 251.
https://arch.astate.edu/all-etd/251
Included in
Biology Commons, Medical Cell Biology Commons, Oncology Commons
