An Automated High-Throughput Phenotyping System for Marchantia polymorpha

Document Type

Article

Publication Title

Methods in molecular biology (Clifton, N.J.)

PubMed ID

35895191

MeSH Headings (Medical Subject Headings)

Gene Editing; Genome-Wide Association Study; Marchantia (genetics)

Abstract

High-throughput phenotyping (HTP) allows automation of fast and precise acquisition and analysis of digital images for the detection of key traits in real time. HTP improves characterization of the growth and development of plants in controlled environments in a nondestructive fashion. Marchantia polymorpha has emerged as a very attractive model for studying the evolution of the physiological, cellular, molecular, and developmental adaptations that enabled plants to conquer their terrestrial environments. The availability of the M. polymorpha genome in combination with a full set of functional genomic tools including genetic transformation, homologous recombination, and genome editing has allowed the inspection of its genome through forward and reverse genetics approaches. The increasing number of mutants has made it possible to perform informative genome-wide analyses to study the phenotypic consequences of gene inactivation. Here we present an HTP protocol for M. polymorpha that will aid current efforts to quantify numerous morphological parameters that can potentially reveal genotype-to-phenotype relationships and relevant connections between individual traits.

First Page

11

Last Page

17

DOI

10.1007/978-1-0716-2537-8_2

Publication Date

1-1-2022

Link to Full Text

Share

COinS