Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells
Document Type
Article
Publication Title
International journal of molecular sciences
PubMed ID
35163062
MeSH Headings (Medical Subject Headings)
Antineoplastic Agents, Phytogenic (pharmacology); Arachis (chemistry); Caspase 9 (metabolism); Cell Line, Tumor; Cell Proliferation (drug effects); Cell Survival (drug effects); Drug Screening Assays, Antitumor; G2 Phase Cell Cycle Checkpoints (drug effects); Gene Expression Regulation, Neoplastic (drug effects); Humans; Inhibitory Concentration 50; Plant Roots (chemistry); Poly(ADP-ribose) Polymerases (metabolism); Stilbenes (pharmacology); Triple Negative Breast Neoplasms (drug therapy, metabolism)
Abstract
Triple-negative breast cancer (TNBC) is unresponsive to typical hormonal treatments, causing it to be one of the deadliest forms of breast cancer. Investigating alternative therapies to increase survival rates for this disease is essential. The goal of this study was to assess cytotoxicity and apoptosis mechanisms of prenylated stilbenoids in TNBC cells. The prenylated stilbenoids arachidin-1 (A-1) and arachidin-3 (A-3) are analogs of resveratrol (RES) produced in peanut upon biotic stress. The anticancer activity of A-1 and A-3 isolated from peanut hairy root cultures was determined in TNBC cell lines MDA-MB-231 and MDA-MB-436. After 24 h of treatment, A-1 exhibited higher cytotoxicity than A-3 and RES with approximately 11-fold and six-fold lower IC, respectively, in MDA-MB-231 cells, and nine-fold and eight-fold lower IC, respectively, in MDA-MB-436 cells. A-1 did not show significant cytotoxicity in the non-cancerous cell line MCF-10A. While A-1 blocked cell division in G2-M phases in the TNBC cells, it did not affect cell division in MCF-10A cells. Furthermore, A-1 induced caspase-dependent apoptosis through the intrinsic pathway by activating caspase-9 and PARP cleavage, and inhibiting survivin. In conclusion, A-1 merits further research as a potential lead molecule for the treatment of TNBC.
DOI
10.3390/ijms23031139
Publication Date
1-20-2022
Recommended Citation
Mohammadhosseinpour, Sepideh; Ho, Linh-Chi; Fang, Lingling; Xu, Jianfeng; and Medina-Bolivar, Fabricio, "Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells" (2022). Arkansas Biosciences Institute. 36.
https://arch.astate.edu/abi/36
