Natural variation in LONELY GUY-Like 1 regulates rice grain weight under warmer night conditions

Authors

Jaspreet Sandhu, University of Nebraska-Lincoln
Larissa Irvin, University of Nebraska-Lincoln
Anil Kumar Chandaran, University of Nebraska-Lincoln
Shohei Oguro, University of Nebraska-Lincoln
Puneet Paul, University of Nebraska-Lincoln
Balpreet Dhatt, University of Nebraska-Lincoln
Waseem Hussain, University of Nebraska-Lincoln
Shannon S. Cunningham, Arkansas Biosciences Institute at A-State
Cherryl O. Quinones, Arkansas Biosciences Institute at A-State
Argelia Lorence, Arkansas Biosciences Institute at A-State
Maria Arlene Adviento-Borbe, Delta Water Management Research Unit, USDA-ARS, Jonesboro, AR 72401, USA.
Paul Staswick, University of Nebraska-Lincoln
Gota Morota, Virginia Polytechnic Institute and State University
Harkamal Walia, University of Nebraska-Lincoln

Document Type

Article

Publication Title

Plant physiology

PubMed ID

38820200

MeSH Headings (Medical Subject Headings)

Oryza (genetics, growth & development, physiology); Plant Proteins (genetics, metabolism); Edible Grain (genetics, growth & development, physiology); Genome-Wide Association Study; Gene Expression Regulation, Plant; Genetic Variation

Abstract

Global nighttime temperatures are rising at twice the rate of daytime temperatures and pose a challenge for rice (Oryza sativa) production. High nighttime temperature (HNT) stress affects rice yield by reducing grain weight, size, and fertility. Although the genes associated with these yield parameters have been identified and characterized under normal temperatures, the genetic basis of grain weight regulation under HNT stress remains less explored. We examined the natural variation for rice single grain weight (SGW) under HNT stress imposed during grain development. A genome-wide association analysis identified several loci associated with grain weight under HNT stress. A locus, SGW1, specific to HNT conditions resolved to LONELY GUY-Like 1 (LOGL1), which encodes a putative cytokinin-activation enzyme. We demonstrated that LOGL1 contributes to allelic variation at SGW1. Accessions with lower LOGL1 transcript abundance had higher grain weight under HNT. This was supported by the higher grain weight of logl1-mutants relative to the wild type under HNT. Compared to logl1-mutants, LOGL1 over-expressers showed increased sensitivity to HNT. We showed that LOGL1 regulates the thiamin biosynthesis pathway, which is under circadian regulation, which in turn is likely perturbed by HNT stress. These findings provide a genetic source to enhance rice adaptation to warming night temperatures and improve our mechanistic understanding of HNT stress tolerance pathways.

First Page

164

Last Page

180

DOI

10.1093/plphys/kiae313

Publication Date

9-2-2024

E-ISSN

1532-2548

Recommended Citation

Sandhu, Jaspreet; Irvin, Larissa; Chandaran, Anil Kumar; Oguro, Shohei; Paul, Puneet; Dhatt, Balpreet; Hussain, Waseem; Cunningham, Shannon S.; Quinones, Cherryl O.; Lorence, Argelia; Adviento-Borbe, Maria Arlene; Staswick, Paul; Morota, Gota; and Walia, Harkamal, "Natural variation in LONELY GUY-Like 1 regulates rice grain weight under warmer night conditions" (2024). Arkansas Biosciences Institute. 1.
https://arch.astate.edu/abi/1