DNAp: A Pipeline for DNA-Seq Data Analysis
Document Type
Article
Publication Title
Scientific Reports
Abstract
Next-generation sequencing is empowering genetic disease research. However, it also brings significant challenges for efficient and effective sequencing data analysis. We built a pipeline, called DNAp, for analyzing whole exome sequencing (WES) and whole genome sequencing (WGS) data, to detect mutations from disease samples. The pipeline is containerized, convenient to use and can run under any system, since it is a fully automatic process in Docker container form. It is also open, and can be easily customized with user intervention points, such as for updating reference files and different software or versions. The pipeline has been tested with both human and mouse sequencing datasets, and it has generated mutations results, comparable to published results from these datasets, and reproducible across heterogeneous hardware platforms. The pipeline DNAp, funded by the US Food and Drug Administration (FDA), was developed for analyzing DNA sequencing data of FDA. Here we make DNAp an open source, with the software and documentation available to the public at http://bioinformatics.astate.edu/dna-pipeline/.
First Page
6793
DOI
10.1038/s41598-018-25022-6
Publication Date
5-1-2018
Recommended Citation
Causey, Jason L.; Ashby, Cody; Walker, Karl; Wang, Zhiping Paul; Yang, Mary; Guan, Yuanfang; Moore, Jason H.; and Huang, Xiuzhen, "DNAp: A Pipeline for DNA-Seq Data Analysis" (2018). Center for No Boundary Thinking. 9.
https://arch.astate.edu/cnbt/9
