Date of Award
3-24-2021
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Elizabeth Hood
Committee Members
Asela Wijeratne; Jianfeng Xu
Call Number
LD 251 .A566t 2020 L35
Abstract
Transgenic plant-based production for industrial enzymes is an evolutionary tool. Green bio- factories have powerful potential to produce desirable product on the most economical and efficient level. Seed itself provides protein a very stable environment, solving the problem of storage. Transgenic plants easily scale up production. Increasing population, climate change and low amounts of arable land create a damaging situation for which a plant-based production system is beneficial. The transgenic plant-based production system rather than microbial fermentation is a solution for this rising scenario. Epigenetics is a natural phenomenon which causes heritable changes in gene expression alternative to the involvement of DNA sequence in protein encoding genes. More precisely it is a change in phenotype without a change in genotype. Epigenetic factors may influence protein accumulation during seed development. Non-coding RNA, DNA methylation and histone modification influence protein coding genes. In this project, transgenic maize expressing a fungal manganese peroxidase gene was generated by using Agrobacterium tumefaciens transformation. With the globulin-1 promoter as a single insert in the genome, transgenic plants were variable in protein production. Expression was improved by backcrossing and high protein producing lines were selected by protein accumulation screening. Greenhouse grown plants were used to harvest embryos and isolate DNA. Genomic DNA was treated with sodium bisulfite, a gold strandard technique for DNA methylation analysis. Next generation sequencing, genomics and transcriptomics are new technologies that may help us understand the potential influence of epigenetic factors on protein accumulation. Differentiated DNA methylation patterns in high and low protein accumulation lines may illustrate effects of epigenetic factors on protein coding genes.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Lal, Akash, "Investigation of the state of DNA methylation in high and low protein accumulating maize lines" (2021). Student Theses and Dissertations. 346.
https://arch.astate.edu/all-etd/346
