Date of Award
3-24-2021
Document Type
Thesis
Degree Name
Agriculture, MSA
First Advisor
Elizabeth Hood
Committee Members
Argelia Lorence; Jianfeng Xu
Call Number
LD 251 .A566t 2020 B66
Abstract
New technologies are vital for growing industries that require large amounts of enzymes at a cheaper production cost. Plant transformation technologies coupled with established cropping systems have allowed us to produce enzymes at more efficient rates than ever before. During breeding, offspring are selected for high production of the enzyme of interest and discontinues lines of low production. This continued range is an observed phenomenon in increasingly isogenic lines, but not fully understood. We hypothesize that distinct populations of non-coding RNAs in isogenic corn lines influence enzyme accumulation in seed. This project sequenced high and low manganese peroxidase (MnP)-expressing families to observe non-coding RNA populations. Bioinformatic analysis has provided non-protein coding RNA sequences and gene transcript counts for MnP gene. Transcript counts of MnP genes compared to protein accumulation indicates other factors influencing accumulation. Unique non-coding RNAs were found and show differences in expression among the expression groups.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Booth, Amber, "Non-coding RNA influence on Manganese Peroxidase Quantity Variation in Isogenic Corn Lines." (2021). Student Theses and Dissertations. 343.
https://arch.astate.edu/all-etd/343
