Date of Award
3-24-2021
Document Type
Thesis
Degree Name
Biology, MS
First Advisor
Gregory Phillips
Call Number
LD 251 .A566t 2020 H35
Abstract
Gametophytic embryogenesis is a method used to achieve homozygosity in plant breeding programs. However, its application is limited within the Fabaceae, which has historically demonstrated morphogenic recalcitrance in comparison to other plant families (i.e., Brassicaceae and Poaceae). Here, the immature male gametophyte — or microspore — was used to investigate gametophytic embryogenesis in soybean (Glycine max [L.] Merrill). In so doing, an induction regimen was optimized to support embryogenic growth through pretreatment temperature shock, a nitrogen starvation medium, and other post-isolation culture conditions. In addition, parallels were drawn between soybean microspore cytology and that which has been reported in model gametophytic embryogenesis systems. Lastly, RNA-Seq was performed to assess the transcriptomic landscape of microspores following pretreatment stress, allowing the identification of candidate genes involved in the induction of microspore totipotency. The findings herein provide insight into gametophytic embryogenesis in the context of microspore embryogenesis and may prove useful in legume breeding programs.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Hale, Bretton M., "Induction And Characterization of Microspore Embryogenesis in Soybean (Glycine Max [L.] Merrill)" (2021). Student Theses and Dissertations. 341.
https://arch.astate.edu/all-etd/341
