Date of Award

8-28-2021

Document Type

Thesis

Degree Name

Molecular Bioscience, MS

First Advisor

Argelia Lorence

Committee Members

Aaron Shew; Arlene Adviento-Borbe; Harkamal Walia; Jianfeng Xu

Call Number

LD 251 .A566t 2021 C86

Abstract

Rice grain size is affected by high nighttime temperature stress (HNT). Although studied in small subsets, the Rice Diversity Panel 1 has not been extensively studied to determine HNT effects on grain size. Accessions have been identified as tolerant and sensitive to HNT under greenhouse conditions (Dhatt et al., 2020). A high throughput phenotyping (HTPP) method and a PlantCV2 workflow were implemented in this work to determine grain dimensions. Seed area was found to be a good indicator of HNT tolerance with a coefficient of variation being 15%. Among tolerant accessions, seed area increased by an average of 1.57 mm2 under HNT stress whereas sensitive accessions resulted in reduced seed area. Seed length and width were not affected by HNT stress. By utilizing HTPP methods, seed architecture of large populations can be analyzed without risking breakage. This study is foundational for elucidating mechanisms of rice chalkiness tolerance under HNT stress.

Rights Management

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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