Date of Award
11-12-2021
Document Type
Thesis
Degree Name
Molecular Bioscience, MS
First Advisor
Fabricio Medina-Bolivar
Committee Members
Argelia Lorence; Asela Wijeratne
Call Number
LD 251 .A566t 2021 G35
Abstract
Abiotic stress, such as drought, causes over five-hundred million dollars in losses in peanut production per year in the world. Peanuts are known to produce antioxidant prenylated stilbenoids, however, their role in abiotic stress tolerance have not been elucidated. The overall goal of this project was to determine if selected prenylated stilbenoids play a role against water stress in peanut. To address this issue, transgenic hairy roots with the capacity to produce diverse prenylated stilbenoids using an RNA-interference (RNAi) approach were established. RNAi molecular constructs targeting a stilbenoid-specific prenyltransferase were designed, synthesized, and expressed via Agrobacterium rhizogenes-mediated transformation in peanut hairy roots. The transgenic hairy roots showed reduced levels of prenylated stilbenoids when compared to wild-type hairy roots. Validation of prenyltransferase AhR4DT-1 knockdown was performed using RT- qPCR. PEG treatment decreased the biomass of the peanut hairy roots and suggested that prenylated stilbenoids play role against water stress in the peanut hairy roots.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Gajurel, Gaurav, "Metabolic Engineering of Peanut Hairy Roots Via RNA Interference to Study the Role of Prenylated Stilbenoids Against Water Stress" (2021). Student Theses and Dissertations. 288.
https://arch.astate.edu/all-etd/288
