Date of Award
2-8-2022
Document Type
Thesis
Degree Name
Molecular Bioscience, MS
First Advisor
Asela Wijeratne
Committee Members
Alison Robertson; Maureen Dolan; Mohamed Milad
Call Number
LD 251 .A566t 2021 P68
Abstract
Phytophthora stem and root rot (PSR), a soybean disease causes annual losses of up to $250 million in the United States alone. Using the Rps (resistant to Phytophthora sojae) gene in soybean is the main management technique. However, Rps genes are only effective until 8-15 years in the field. Therefore, further understanding of the Rps gene regulation and immune response in soybean is desperately needed. In this study, first, three commercial DNA methylation detection kits were examined on soybean (cultivar Williams 82,which carries Rps1k) DNA samples. All three kits provided libraries with excellent quality and coverage. Secondly, the differential DNA methylation in P. sojae-inoculated and non-inoculated soybean samples was detected. Mostly, methylated cytosines were discovered at CpG context then CHG and CHH. The highest differentially methylated cytosines were found in promoter region and were associated with genes involved in acyltransferase activity and ATP binding. In conclusion, this study demonstrations the changes in differential DNA methylation in P.sojae infected soybean.
Rights Management
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Poudel, Shyaron, "Investigation Of the Methylome Pattern Changes in Phytophthora Sojae Infected Soybean (Glycine Max)" (2022). Student Theses and Dissertations. 274.
https://arch.astate.edu/all-etd/274
